Non-injection methods of skin biorevitalization

Non-injection methods of skin biorevitalization (from the Greek “bios” – life and the Latin “revitalis” – revitalization) – is the process of restoring and activating skin functions at the histological level.

• restores water balance
• improves cellular activity and strengthens skin structures
• restores a healthy appearance
• slows down aging processes

What is biorevitalization for?

• Increased collagen and elastin production
• Acceleration of cell renewal
• Normalization of melanocyte activity
• Increase in hyaluronic acid
• Reduction of oxidative stress
• Restoration of microcirculation
• Normalization of sebaceous gland function
• Restoration of mitochondria
• Increase in fibroblasts and other dermal cells
• Control of telomere length

Restoring fibroblast function

• Extracellular matrix (ECM) synthesis – Production of collagen, elastin, proteoglycans to maintain tissue structure.
• ECM remodeling – Destruction of old matrix and its renewal through matrix metalloproteinases (MMPs).
• Regulation of inflammation – Secretion of cytokines and chemokines to recruit immune cells.
• Wound healing – Proliferation, migration and differentiation into myofibroblasts to repair damage.
• Angiogenesis – Stimulation of new vessel growth through VEGF.
• Immunomodulation – Effect on the activity of macrophages, T-lymphocytes and other immune cells.

Restoration of the dermoepidermal junction

Dermoepidermal junction (DEJ) is a structure that unites the epidermis and the dermis, ensuring their strong adhesion and functional interaction.

• Basement membrane: A thin plate composed of collagen and glycoprotein components that separates the epidermis and dermis.
• Anchor fibrils: Collagen fibers that attach the basement membrane to the dermis, providing mechanical strength to the connection.

Functions of the DEJ:

• Mechanical support
• Transport of substances
• Regulation of cellular activity
• Transport of substances
• Regulation of cellular activity

What components are required for recovery?

• Amino acids: building blocks for the synthesis of collagen and elastin.
• Peptides: stimulate cellular activity and regeneration processes.
• Vitamins: provide antioxidant protection and improve metabolic processes in the skin.
• Minerals and trace elements: support cellular metabolism and skin health
• Hyaluronic acid: helps retain water molecules, ensuring skin hydration.
• Antioxidants: protect cells from free radical damage.
• Glycerin: enhances moisturizing properties and helps retain moisture in the skin.
• Polynucleotides: accelerate the processes of regeneration and tissue repair.
• Coenzymes: participate in the energy metabolism of cells, contributing to their renewal.

Injection biorevitalization

Method of administration: The drug is injected directly into the deep layers of the skin using thin needles or cannulas.
Effectiveness: Provides deep penetration of active substances, which leads to a more pronounced and faster result.
Number of procedures: Usually 2-5 sessions are required with an interval of 2-4 weeks to achieve the desired effect.
Advantages: Fast results, possibility of spot treatment on problem areas.
Disadvantages: Possible discomfort, small bruises or papules after the procedure; recovery time is needed.

Non-injection biorevitalization

Method of administration: Active ingredients are delivered into the skin using microneedling devices without disrupting the integrity of the skin. Chemical peels. Facial procedures.
Effectiveness: Less deep penetration compared to the injection method; the effect may be less pronounced and require more procedures.
Number of procedures: Usually 5-10 sessions are required with a frequency of 1-2 times a week to achieve the desired result.
Advantages: Painlessness, no scars and no recovery period after the procedure.
Disadvantages: More treatments may be required to achieve the same effect; less pronounced results compared to the injection method.

The role of non-injection methods in modern cosmetology

• Non-injection methods are safe and effective
• Minimal risk, fast recovery
• Convenience of use
• Natural results
• Can stay socially active

Non-injection biorevitalization methods

• Microneedling (dermapen, mesoroller; needle length 0.25-1.5 mm depending on the treatment area)
• Acid peels
• Device-based techniques (without damaging the integrity of the skin)
• Facial procedures (restoration of the skin’s water balance + skin lipid mantle)

Microneedling

Mechanism of triggering processes in skin cells during microneedling:

1. Injury and inflammation phase

• Mechanical skin damage (microchannels into which the mesococktail penetrates, microruptures of collagen and elastin fibers)
• Activation of the inflammatory response (release of cytokines: interleukins and tumor necrosis factors) + immune system cells are activated (macrophages, neutrophils)
• Release of growth factors (Platelets secrete growth factors (TGF-β, PDGF, VEGF), which trigger tissue regeneration)

2. Proliferation phase (1-7 days after the procedure):

• Fibroblast activation (Fibroblasts migrate to damaged areas and begin to actively produce collagen, mainly young collagen type III, elastin and hyaluronic acid)
• Neoangiogenesis (due to vascular endothelial growth factor (VEGF))
• Increased cellular activity (active division of keratinocytes, filling microchannels)

3. Remodeling phase (4-6 weeks):

• Collagen maturation:
• Type III collagen is gradually replaced by stronger type I collagen.
• The dermal matrix is ​​strengthened, the skin becomes more elastic and dense.
• Inflammation reduction:
• Inflammatory processes are completed, the skin restores barrier functions.
• Tissue regeneration:
• Fine wrinkles, scars and stretch marks are eliminated due to the renewal of the dermis.

Results of the procedure:

Improvement of skin texture
Increase in elasticity and hydration
Evens out skin tone
Narrowing of pores

Biorevitalizing cocktail HA+ for prevention and correction of age-related changes
Comprehensive approach: replaces several procedures at once thanks to its multifunctional composition.

Effective for:

• Prolonged hydration
• Increase skin firmness and elasticity
• Brightening and smoothing out the skin surface
• Acne scars correction and recovery after aggressive procedures

Ingredients:

• Hyaluronic acid 1.6 million Da
• 17 amino acids
• Vitamins A, C, E, PP, B1, 6, 8, 12
• Microelements

Sterile cocktails are intended for external use – microneedling, device-based procedures. Such preparations are successfully and widely used by many dermatologists and cosmetologists for mesotherapy practices.

17 amino acids improve the texture and elasticity of the dermis 

1. Glycine
2. Proline
3. Isoleucine
4. Histidine HCl
5. Alanine
6. Arginine HCl
7. Leucine
8. Lysine HCl
9. Methionine
10. Phenylalanine
11. Serine
12. Threonine
13. Valine
14. Tryptophan
15. Glutamine
16. Taurine
17. Hydroxyproline

Hyaluronic acid

• non-crosslinked hyaluronic acid
• high molecular weight 1.6 million Da
• provides restoration of physiological homeostasis in the intercellular substance
• restoration of the fraction of large HA molecules

Methionine, histidine and niacinamide neutralize free radicals

Vitamin B complex (biotin, thiamine, pyridoxine) catalyzes biochemical reactions of protein, fat and neurotransmitter synthesis, supports the physiological state of skin cells and accelerates their regeneration

Trace elements – calcium and potassium – support the connection in the dermo-epidermal junction, ensuring the stability of the keratin filament network

• Calcium triggers the production of protein kinase C, which stimulates the differentiation and proliferation of basal keratinocytes.
• Potassium participates in the work of the sodium-potassium pump (Na+/K+- ATPase) – a mechanism for the distribution of ions and control of water content in cells, prevents dehydration and stable intercellular signal transmission, maintains the pH of the skin, protecting it from the development of pathogenic microflora.

MICRONEEDLING PROTOCOL

1. Cleansing according to skin type (P171, P172+ P174, P175) Treat the skin with antiseptic.
2. Apply 2.5 ml of Biorevitalizing Cocktail HA+ to the treated area. Smoothly apply dermapen to the skin, evenly distributing the product.
3. Active mask (P135 Hydra-Help Mask + alginate mask P208/P106)
4. Finishing cream: Н210 Cream-Protector SPF30/ Н218 Sun-Block Emulsion SPF50 / Р140 Recovery Cream.

HAIR+ Restorative Cocktail for Stimulating Hair Growth

Effective for:

• Complex treatment of alopecia
• Prevention of hair loss
• Strengthening thin and brittle hair
• Control of oiliness

Ingredients:

• Capixyl peptide complex
• Vitamins B1, B6
• sh-Oligopeptide-4
• Hydrolyzed keratin, panthenol

Sterile cocktails are intended for external use – microneedling, device-based procedures. Such preparations are successfully and widely used by many dermatologists and cosmetologists for mesotherapy practices.

Capixyl Peptide Complex

It is a biomimetic peptide (acetyl tetrapeptide-3) combined with red clover extract rich in biochanin A. It has anti-inflammatory effects. It directly affects 5-alpha reductase, regulating dihydrotestosterone (DHT) levels. It improves the integrity of extracellular matrix (ECM) proteins.

Synergy of preventive home care

ANTI HAIR LOSS CONCENTRATE

NOT ADDICTIVE

• Procapil
• Nucleo Tricho-hyal
• Polyquaterniums
• Rosemary oil and olive oil

SCALP PEELING

Soft, gentle cleansing of the skin, without irritation, even for sensitive scalps

• Peppermint oil
• Cinnamon oil
• Chamomile flower extract
• Urea
• Allantoin
• Panthenol

Device-based techniques with the biorevitalizing cocktail NA+ for the prevention and correction of age-related changes

HydraFacial

At stage 3, replace the serum with a mesococktail to moisturize and nourish the skin with active ingredients, thanks to vacuum exposure and hydrodermabrasion. This method is non-invasive and gentle, which allows you to evenly nourish the tissues.

Electroporation

Apply a sterile mesococktail, water-based, to the skin + exposure to short pulses of electric current, which temporarily open aquaporins in cell membranes, allowing active substances to penetrate into the deeper layers of the skin.

Biorevitalizing peels

Mechanism that triggers cellular processes during chemical peels:

• Chemical skin damage: causes denaturation of proteins in cells, dissolves intercellular junctions, damages epidermal cells
• Initiation of inflammatory response: activates immune response. Macrophages and neutrophils migrate to the site of injury. Inflammatory mediators (cytokines, prostaglandins) are released, which leads to swelling, redness and pain
• Activation of growth factors: Local skin damage initiates the release of growth factors, such as TGF-β (transforming growth factor beta), PDGF (platelet-derived growth factor), which stimulate tissue regeneration and healing

PROLIFERATION PHASE + REMODELING PHASE ARE SIMILAR

Level of work in tissues – layers of skin

• cell renewal process
• chemical transformations – release of BAS

Revitalizing peels – “injection-free biorevitalization” – chemical peels, which, in addition to a combination of acids, contain active biorevitalization components.

EXPECTED EFFECT FROM BIOREVITALIZING PEELING

• No downtime
• Instant result immediately after the procedure (brightening, moisturizing, smoothing out the skin surface)

PEEL-EXPERT LIGHT

М304

Skin revitalization without downtime

Start of peel course for all skin types!

• accelerates tissue regeneration
• mild keratolytic effect
• stimulation of keratinocytes and fibroblasts
• restoration of NMF of the epidermis

Possibility of use in the area around the eyes

Ingredients:

Mandelic Acid 15%
Glycolic Acid 6%
Lactic Acid 5%
Salicylic Acid 1.5%
Arginine
Allantoin
рН 3,3 Gel

Glycolic Acid + Arginine

Arginine – an amino acid has a positive charge, when it hits the skin it becomes negative, which contributes to deeper penetration of glycolic acid into the skin. Soothes, moisturizes, accelerates skin regeneration.
Glycolic acid + arginine form an amphoteric complex that has the ability to change charge, giving or adding H+.

• Reduces the irritating ability of glycolic acid – the ability to use even for sensitive skin
• Provides a slow release of components

PEEL EXPERT SHINE

М312

Skin Restructuring

Peel without downtime

• Melanogenesis control
• Skin cell renewal, lifting
• Fibroblast stimulation
• Antioxidant cell protection
• Powerful restoration of skin’s water balance
• Can be used in intimate areas, armpits

Ingredients:

Lactic acid 9%
Mandelic acid 4%
Kojic acid 1%
Glutathione
Retinol 0.25%
4-butylresorcinol
Complex of fermented oils (apricot kernel, sweet almond)
Licorice root extract
рН 3,0 Gel

TCA BIOREVITALIZING PEELING – TC-CONTROL TCA+

Derma-epidermal stimulator
The ideal method to restore optimal physiological qualities of the skin!
Medical product that has
Superficial chemical peel without downtime

INDICATIONS:

• In the complex treatment of acne
• Superficial cicatricial changes
• Pigmentation and dyschromia
• Age-related changes

INGREDIENTS:

• Stabilized trichloroacetic acid 12%
• Mandelic acid 5%
• Kojic acid 5%
• pH 0.2-1, Gel

Packaging: 5 bottles of 5 ml

• a combination of acids stimulates the production of anchor protein fibrils, strengthening the dermato-epidermal contact
• a powerful keratolytic, evens out skin tone and skin surface
• a pronounced lifting effect
• has antimicrobial and anti-inflammatory effects
• blocks the tyrosinase enzyme

4-step universal peel protocol

1. Cleansing + toning according to skin type. Remove excess oil from the skin with M303 Pre-peel lotion AHA & BHA (differential diagnosis)
2. Application of PEEL-EXPERT or TC-СONTROL TCA+ peel with a brush or cotton swabs. Exposure time: 2-5 minutes (depending on the skin reaction). If necessary, additional layers can be applied to enhance the effect. Neutralization M311 Neutralizer
3. Active mask – P135 Hyrda-help mask, exposure 10-15 min, lightly introduce, blot the remains with a tissue.
4. Р218 Sun-block emulsion SPF 50 / H210 Cream-protector SPF 30

HYDRA-HELP MASK

Do not wash off! Press in lightly.

• Restores skin’s water balance
• Long-lasting deep moisturizing effect (aquaporins!)
• For dry, dehydrated, hypersensitive skin
• After aggressive procedures
• In the event of climate change and temperature conditions

The main agent is the biotechnological complex Aquaxyl from two plant sugars that act synergistically. Aquaxyl restores the level of hydration in the skin, acting on the principle of continuous hydration and restructuring.

• increases the level of aquaporins
• activates the synthesis of its own ceramides
• normalizes desquamation of horny scales
• improves skin surface
• instantly gets rid of signs of skin peeling
• reduces the level of transepidermal moisture loss
• increases the level of hydration of the deep layers
• of the epidermis

SPF products

UV PROTECTION + MOISTURIZATION, ANTIOXIDANT PROTECTION

H210 Cream-protector spf30 – thick texture, for normal skin prone to dryness and sensitivity

• Ectoine
• Titanium dioxide
• Chemical filters (UVA/UVB) of the latest generation

H218 Sun-block emulsion spf50 – ultra-light texture for combination and oily skin.

• Matmarine complex 3%
• Glycerin
• UVA/UVB filters

MANDATORY FOR POST-PEEL HOME CARE!

Mechanism of action of topical agents

Adsorption of active substances into the stratum corneum through microcracks, pores and intercellular spaces

Hyaluronic acid binds water molecules, creating a moisturizing barrier that retains moisture in skin cells + activates the synthesis of other glycosaminoglycans

• Restoration of barrier function and improvement of skin texture
• Activation of cellular metabolism, acceleration of cell regeneration
• Neutralization of free radicals, reduction of inflammation and aging

Peptides that transform growth factors affect cell membranes and improve permeability for other molecules, increasing the efficiency of penetration of substances into the deeper layers of the dermis

Amino acids strengthen keratinocytes, helping to restore and maintain the barrier functions of the skin + stimulation of fibroblasts

BIOREVITALIZING CARE WITH MYORELAXING EFFECT

Р117 ANTI-WRINKLE SERUM  (Syn-ake, hyaluronic acid)

• Botulinum-like effect
• Moisturizing
• Smoothing fine wrinkles

P135 HYDRA-HELP MASK (Aquaxyl, hyaluronic acid)

• As a shield for an alginate mask
• Restoration of the skin’s hydrobalance
• Long-lasting deep moisturizing effect
• For dry, dehydrated, hypersensitive skin

Р208 ANTI-WRINKLE MASK (Homeostatine™ m.s., dictyopteris oil, acetylhexapeptide 8, alginate)

• Triple action on wrinkles: Smoothing, filling, lifting
• Moisturizing and mineralizing the dermis
• Prolonging the effect of botulinum therapy

Р140 RECOVERY CREAM  (polynucleotides, hyaluronic acid)

• Stimulation of the skin’s own DNA restoration
• Activation of metabolic processes in cells
• Intensive hydration

SYNTHETIC BOTOX PEPTIDES

ARGIRELINE (acetil hexapeptide-8)

• the first hexapeptide that mimics the N-terminal end of SNAP-25.
• Argireline competes with this protein for a place in the SNARE complex. A short protein consisting of a series of amino acids, it eliminates and smoothes wrinkles in a non-invasive way, without resorting to injections.

SYN-AKE (Dipeptide diaminobutyroyl benzylamide diacetate)

• blocks the muscle nicotinic acetylcholine receptor mnAChR on the postsynaptic membrane of the muscle cell.
• prevents the release of NA+ ions
• depolarization does not occur
• the muscle cell does not contract

COMPLEX EFFECT OF BOTOX-LIKE PEPTIDES FOR MAXIMUM BOTOX-LIKE EFFECT

BIOREVITALIZING CARE WITH MYORELAXING EFFECT

1. Cleansing P172/ P173 + P174/P175
2. Exfoliation\enzyme therapy P200 Soft-peeling enzyme mask, wash off with water.
3. Massage Р102, toning P174/P175
4. Р117 ANTI-WRINKLE SERUM
5. Р135 HYDRA-HELP MASK + Р208 ANTI-WRINKLE MASK
6. Targeted action serum
7. Р204 Matrix eye cream
8. Р140 Recovery cream

BIOREVITALIZING CARE WITH MYORELAXING EFFECT+ MICRONEEDLING

1. Cleansing P172/ P173 + P174/P175
2. Exfoliation Р200 Soft-peeling enzyme mask, wash off with water.
3. Apply 2.5 ml of Biorevitalizing Cocktail NA+ to the treated area. Use smooth movements to treat the skin with the dermapen, evenly distributing the product.
4. Р135 HYDRA-HELP MASK + Р208 ANTI-WRINKLE MASK
5. Targeted action serum: Р117 ANTI-WRINKLE SERUM  / Р179 ANTI-RED CALMING SERUM
6. Р204 Matrix eye cream
7. H210/H218 or Р140 Recovery cream

BIOREVITALIZING CARE WITH MYORELAXING EFFECT + TC-CONTROL TCA+ peeling

1. Cleansing P172/ P173 + P174/P175
2. Exfoliation Р200 Soft-Peeling Enzyme Mask, wash it off.
3. Degrease the skin with M303 Pre-Peel Lotion AHA & BHA (differential diagnostics)
4. Apply PEEL-EXPERT or TC-СONTROL TCA+ peel with a brush or cotton swabs. Exposure time: 2-5 minutes (depending on the skin reaction). If necessary, additional layers can be applied to enhance the effect. Neutralization M311 Neutralizer
5. Р135 HYDRA-HELP MASK + Р208 ANTI-WRINKLE MASK
6. Targeted action serum: Р117 ANTI-WRINKLE SERUM  / Р179 ANTI-RED CALMING SERUM
7. Р204 Matrix Eye Cream
8. H210/H218 cream or Р140 Recovery Cream

How to combine methods to achieve the best results

• A competent combination of procedures reduces recovery time and increases efficiency.
• Comprehensive courses give a prolonged effect, not just an instant improvement.
• Individual protocol – the key to the result
• The choice of techniques depends on the skin condition and age of the patient.
• A phased approach minimizes rehabilitation and gives a lasting effect.

Gradual increase in the acid effect of Peel-Expert Light, Shine, then TC-CONTROL TCA+

In the intervals between procedures, we carry out care procedures with a moisturizing, restorative, corneotherapy effect

Microneedling is best done 10-14 days before/after peeling

BIOREVITALIZATION: the key to rejuvenation

Complex biorevitalization is a strategic method of combating skin aging that works on all levels: from hydration to collagen stimulation and cell renewal

revitalization ≠ just hydration

• restoration of barrier functions, stimulation of fibroblasts, prevention of photoaging
• improves hydration, elasticity and texture of the skin
• launches natural regeneration processes
• surface peels and acids enhance the penetration of active ingredients
• microneedling stimulates collagen production and increases the effectiveness of biorevitalizers.

Deep revitalization – not only hyaluronic acid

• Peptides, amino acids, antioxidants enhance cellular renewal
• Modern formulas work with turgor, tone, vascular network